Sustained and controlled release of

20 Materials which undergo self-assembly to form supermolecular structures can provide 21 alternative strategies to drug loading problems in controlled release application. RADA 16 is 22 a simple and versatile self-assembling peptide with a designed structure formed of two 23 distinct surfaces, one hydrophilic and one hydrophobic that are positioned such a well24 ordered fashion allowing precise assembly into a predetermined organization. A “smart” 25 architecture in nanostructures can represent a good opportunity to use RADA16 as a carrier 26 system for hydrophobic drugs solving problems of drugs delivery. In this work, we have 27 investigated the diffusion properties of Pindolol, Quinine and Timolol Maleate from 28 RADA16 in PBS and in BSS-PLUS at 37 °C. A sustained, controlled, reproducible and 29 efficient drug release has been detected for all the systems, which has allow to understand the 30 dependence of release kinetics on the physicochemical characteristics of RADA16 structural 31 and chemical properties of the selected drugs and the nature of solvents used. For the analysis 32 various physicochemical characterization techniques were used in order to investigate the 33 state of the peptide before and after the drugs were added. Not only does RADA16 optimise 34 drug performance, but it can also provide a solution for drug delivery issues associated with 35 lipophilic drugs. 36